Thermo Scientific™ Vivid™ Green Fluoreszenz-Standard

Description

Vivid™ Fluorescent Standards represent the products formed during oxidation of Vivid™ Substrates by cytochrome P450 enzymes. These standards enable quantification of enzyme activity when used as part of the Vivid™ CYP450 Screening Kits. Vivid™ Fluorescent Standards are included in the Vivid™ Screening Kits, but may also be purchased separately. They are supplied as a dried film.

More About Vivid™ CYP450 Screening Kits

Vivid™ CYP450 Screening Kits are high-throughput fluorescence-based assays for detection of enzyme-drug interactions and CYP450 inhibition. Vivid™ CYP450 Screening Kits include Vivid™ Substrate, Vivid™ Fluorescent Standard, reaction buffer, Vivid™ Regeneration System, NADP+, and CYP450 BACULOSOMES™ Plus Reagent. Vivid™ Fluorogenic Substrates are blocked dyes that yield minimal fluorescence signal until cleaved or hydroxylated. Oxidation at either of two potential sites releases the highly fluorescent product. CYP450 BACULOSOMES™ Plus Reagents are microsomes prepared from insect cells expressing a human CYP450 isozyme and human cytochrome P450 reductase. CYP450 BACULOSOMES™ Plus Reagents offer a distinct advantage over human liver microsomes in that only one CYP450 isozyme is expressed, thereby preventing metabolism by other CYP450s.

Applications

• High-throughput screening of enzyme-drug interactions
• Compound profiling for drug inhibition of cytochrome P450 isozymes
• Generation of predictive SAR models to guide compound acquisition