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Macherey-Nagel™ NucleoBond™ CB 20/CB 100, Midi kit for high integrity DNA from cells and blood
Anion exchange chromatography for purification of DNA from whole blood and cultured cells.
139.00 EUR - 237.00 EUR
Specifications
| Format | Mini Gravity-flow Column |
|---|---|
| Isolation Technology | Anion Exchange Chromatography Technology |
| For Use With (Application) | Applications: Genomic DNA from whole blood, serum, plasma, cultured cells, buffy coat. |
| No. of Reactions | 20 preps |
| Product Type | NucleoBond™ CB |
| Product Code | Brand | Yield | Column Type | Price | Quantity & Availability | ||||
|---|---|---|---|---|---|---|---|---|---|
| Product Code | Brand | Yield | Column Type | Price | Quantity & Availability | ||||
|
10121251
|
Macherey-Nagel™
740508 |
100 μg | NucleoBond AXG 100 |
237.00 EUR
20 reactions |
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|
11922452
|
Macherey-Nagel™
740507 |
5-20 μg | NucleoBond AXG 20 |
139.00 EUR
20 reactions |
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Description
Genomic DNA quality is extraordinarily high. Samples are first lysed under special conditions for removal of erythrocytes. After incubation with proteinase K, the sample is diluted with buffer N2 before loading onto the NucleoBond™ column. After elution with high-salt buffer the genomic DNA is precipitated with isopropanol. The DNA purified by this method is suitable for all kinds of subsequent reactions. Kit components: NucleoBond™ CB 100 kit - NucleoBond AXG 100 columns, buffers, proteinase K. NucleoBond™ CB 20 kit - NucleoBond™ AXG 20 columns, buffers, proteinase K.- Anion exchange technology
- Format: gravity flow columns
- Sample material: whole blood, buffy coat, cultured cells (up to 20 mL blood)
- Fragment size: 500bp to 300kb
- Yield: 100μg or 5-20μg depending on column used
- Binding capacity: 100μg or 5-20μg depending on column used
- Preparation time: 4 to 5hrs For purification of up to 500μg genomic DNA from whole blood.
Specifications
| Mini Gravity-flow Column | |
| Applications: Genomic DNA from whole blood, serum, plasma, cultured cells, buffy coat. | |
| NucleoBond™ CB | |
| 4-5hr. |
| Anion Exchange Chromatography Technology | |
| 20 preps | |
| 500kbp |
Safety and Handling
Hazard Category
- Respiratory sensitiser Category 1
- Skin sensitiser Category 1
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