Invitrogen™ Human Thyroid Peroxidase ELISA Kit

Beschreibung

The Human Thyroid Peroxidase ELISA quantitates Hu Thyroid Peroxidase in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Thyroid Peroxidase. Principle of the method The Human Thyroid Peroxidase solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Dieses Gen kodiert ein membrangebundenes Glykoprotein. Das kodierte Protein fungiert als Enzym und spielt eine zentrale Rolle in der Schilddrüsenfunktion. Das Protein trägt zur Iodierung von Tyrosinresten in der Thyroglobulin- und Phenoxy-Esterbildung zwischen Paaren iodinierter Tyrosine bei, um die Schilddrüsenhormone Thyroxin und Triiodthyronin zu erzeugen. Mutationen in diesem Gen sind mit mehreren Störungen der Schilddrüsenhormonogenese verbunden, darunter angeborene Hypoparathyreoidismus, angeborener Struma und Schilddrüsenhormon-Organifikations-Defekt IIA. Für dieses Gen wurden mehrere Transkriptvarianten festgestellt, die unterschiedliche Isoformen kodieren. Weitere Spleißvarianten wurden beschrieben, ihre biologische Natur jedoch nicht bestimmt.